Background : Transforming growth factor-,81 (TGF- /31) has been shown to promote tissue repair and have inumunosuppressive actions, and has been proposed to have a role in rheumatoid arthritis (RA). In this study, we have analyzed the association of five known polymorphisms in TGF-131 gene with RA.
Methods : We analyzed single nucleotide polymorphisms (SNPs) of TGF-131 gene in RA patients (n=110) and controls (n=148). Allele frequencies of SNPs of TGF- /31 gene at positions -800 G/A, -5(>J CA, 869 T/C (LION, 915 G/C (R25I´), and 1628 C/A (´I265I) were determined by PCR-RFLP using genomic DNA.
Results : There were no statistically significant differences in genotype and allele frequency distributions between cases and controls for -509 C/T or 8G9 T/C (L10P) polymorphism (p=0.47 and 0.46, respectively). Analysis of combined -509 C/T and 869 T/C genotype showed that the frequency of heterozygote/heterozygote combination was higher in RA partients which was not statistically significant. In the recessive model, the frequency of polymorphic homozygotes at -509 C/T and 869 ´I/C was lower in male patients with RA (14.3% vs. 31.3%, p=0.16). The frequency of rare alleles at -800 G/A and 915 G/C was very low and was not associated with RA. 1628 C/A (´I265D was not polymorphic in both groups.
Conclusion : The present data indicated that the 5 selected SNPs of TGF-131 gene were not associated with the susceptibility to RA.(Korean J Med 65:335-342, 2003)
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